grnfluoresceblg - Network

Awesome work from Rotem Sorek lab in our department:Rotem's lab & others found this past decade >150 bacterial defense mechanisms...

Awesome work from Rotem Sorek lab in our department:
Rotem's lab & others found this past decade >150 bacterial defense mechanisms against phages.
This new work, led by Erez Yirmiya, explores phage *anti-defense* mechanisms, and it's really cool!
#bacteria #bacteriophage #phages

https://www.nature.com/articles/s41586-023-06869-w

An interesting finding from Tim Bredy, Rob Spitale & others:lncRNAs localize to synapses, regulate synaptic activity and...

An interesting finding from Tim Bredy, Rob Spitale & others:
lncRNAs localize to synapses, regulate synaptic activity and behavior!
#lncRNA #RNA #synapse

https://www.nature.com/articles/s41467-023-43535-1

Another cool-looking paper on #tunnelingnanotubes in #cancer by Emil Lou https://elifesciences.org/articles/85383#content

Another cool-looking paper on #tunnelingnanotubes in #cancer by Emil Lou

https://elifesciences.org/articles/85383#content

A nice short review on #tunnelingnanotubes by Chiara Zurzolohttps://www.oaepublish.com/articles/evcna.2023.05

A nice short review on #tunnelingnanotubes by Chiara Zurzolo

https://www.oaepublish.com/articles/evcna.2023.05

"The mechanism of mRNA activation"A preprint looking at the role of eIF4F in translation initiation at single molecule...

"The mechanism of mRNA activation"
A preprint looking at the role of eIF4F in translation initiation at single molecule resolution.
#mRNA #translation #ribosome #singlemolecule #microscopy

https://www.biorxiv.org/content/10.1101/2023.11.15.567265v1?rss=1

And a new preprint from from Ryan Flynn, Caroline Bertozzi on #glycoRNA Chemical/molecular oriented, they characterize the chemical...

And a new preprint from
from Ryan Flynn, Caroline Bertozzi
on #glycoRNA
Chemical/molecular oriented, they characterize the chemical modification on the RNA that allow N-glycan binding.
"The modified RNA base acp3U is an attachment site for N-glycans in glycoRNA"
https://www.biorxiv.org/content/10.1101/2023.11.06.565735v1

This looks like a very interesting #preprint @biorxivpreprint from Christine Mayr lab:"The FXR1 network acts as signaling scaffold for...

This looks like a very interesting #preprint @biorxivpreprint from Christine Mayr lab:

"The FXR1 network acts as signaling scaffold for actomyosin remodeling"
#RNA #mRNA #RNABindingProtein #microscopy

https://www.biorxiv.org/content/10.1101/2023.11.05.565677v2

<<evil which directly affects me and the ones I love. I tried to persuade them over that taking a stand against Hamas should not be...

<<
evil which directly affects me and the ones I love.
I tried to persuade them over that taking a stand against Hamas should not be controvetial.
But to no avail

It was a really good experience up till now, and I will miss it, and the people I worked with.

2 wks ago I resigned from the editorial board of @bioprotocolbyte .I did so because, in their words "it might not be appropriate for us...

2 wks ago I resigned from the editorial board of @bioprotocolbyte .
I did so because, in their words "it might not be appropriate for us as an organization to make broad statements on social media regarding the ongoing situation, or send out a mass email to the BP community."
I have been tgere since 2016. I truly believe in their mission to help with “The reproducibility crisis”.
So, I am really sad to leave.
But I cannot work for an organization that remains silent in the face of true evil>>

🎯🐠 I've been away for a while. But here:I wrote a blog post on "Imaging mRNA modifications in situ"Featuring:...

🎯🐠 I've been away for a while. But here:
I wrote a blog post on "Imaging mRNA modifications in situ"

Featuring: m6AISH-PLA, ARPLA (on #glycoRNA) & DART-FISH.

#smFISH #m6A #RNA #mRNA #RNAmodifications #microscopy #imaging #singlemolecule

https://greenfluorescentblog.wordpress.com/2023/10/31/imaging-mrna-modifications-in-situ/

Help! I have a problem with #IVT in vitro transcription:I created a set of plasmids with T7 promoter & poly-A tail. my gene (~4kb) is...

Help! I have a problem with #IVT in vitro transcription:
I created a set of plasmids with T7 promoter & poly-A tail. my gene (~4kb) is inserted in between. I have several variations of the same gene. In all cases, I get 2 products: the correct one and a shorter (~2.8kb) one.
Any idea why? How do I solve it?

I use the HiScribe T7 High Yield RNA Synthesis Kit (NEB) with 3"-O-Me-m7G(5")ppp(5")G RNA Cap Structure Analog.
IVT for GFP works well with just one product of correct size.

A letter published @sciencemagazine about the dire political situation in Israel and its effect on academia...

A letter published @sciencemagazine about the dire political situation in Israel and its effect on academia

#No_Democracy_No_Academia

https://www.science.org/doi/10.1126/science.adk3054

>>In addition, we are going translational, and moving to animal work in mice in order to study the role of this process in vivo, and...

>>In addition, we are going translational, and moving to animal work in mice in order to study the role of this process in vivo, and to deveolpe therapy based on this process.
Hopefully, we'll have initial animal data within a few weeks.

So, if you are interested in doing a PhD in our lab, please contact me or Jeff via email.

🔥 📣 This is probably bad timing, with all the political chaos in Israel, but we're looking for a PhD student to continue a grad...

🔥 📣 This is probably bad timing, with all the political chaos in Israel, but we're looking for a PhD student to continue a grad student's project on mRNA transfer through tunneling nanotubes.
Start date ~Jan 2024.
We are looking for someone interested in deciphering the molecular mechanism of mRNA transfer, by performing a CRISPR/siRNA/drug screen, and using molecular biology & biochemistry tools.
(more next post >>)

#mRNA #RNA #mRNALocalization #TunnelingNanotubes

https://www.weizmann.ac.il/molgen/Gerst/research-activities/cell-cell-transfer-mrna-and-its-role-mammalian-cell-physiology

Happy #RNA day!

Happy #RNA day!

Does anyone know what is this white stuff on my tomato plant?

Does anyone know what is this white stuff on my tomato plant?

🔥 The research Elena Conti shared at #EESRNA meeting is published @genesdev It's very interesting and might be relevant to our work...

🔥 The research Elena Conti shared at #EESRNA meeting is published @genesdev

It's very interesting and might be relevant to our work on transperons.

"Nuclear mRNPs are compact particles packaged with a network of proteins promoting RNA–RNA interactions"

#RNA #mRNA #NuclearExport
#yeast

https://genesdev.cshlp.org/content/37/11-12/505.full

The deadline for abstract submission ⬆️ is two weeks away! Submit by July 30!

The deadline for abstract submission ⬆️ is two weeks away! Submit by July 30!

This is cool:"Direct sequencing of total Saccharomyces cerevisiae tRNAs by LC–MS/MS"#RNA #tRNA #MassSpec #RNAmodification #yeast...

This is cool:
"Direct sequencing of total Saccharomyces cerevisiae tRNAs by LC–MS/MS"

#RNA #tRNA #MassSpec #RNAmodification
#yeast

https://rnajournal.cshlp.org/content/29/8/1201.full

Wow! 💥 🔥 🔥 🔥 This is so cool! In this paper, "Spatially resolved single-cell translatomics at molecular resolution",...

Wow! 💥 🔥 🔥 🔥
This is so cool!
In this paper,
"Spatially resolved single-cell translatomics at molecular resolution", they used a tri-probe FISH system combined with in situ amplification and in situ sequencing to detect multiplex translating mRNAs at sub-cellular resolution in cell culture (981 genes) and mouse brain tissue slices (5413 genes!).

#mRNALocalization #mRNA #translation #smFISH #FISHseq

https://www.science.org/doi/10.1126/science.add3067